Quantitative Immunoelectrophoresis of Panicum Mosaic Virus and Strains of St. Augustine Decline

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Abstract
Panicum mosaic virus (PMV), including 7 isolates of St. Augustine decline (SAD) strain, was characterized by rocket quantitative immunoelectrophoresis (QIEP) utilizing rabbit antisera. The titer of each strain in a common host could be quantified by QIEP. The slope of the linear regression between log peak area of the rockets and log-relative concentration of plant sap serial dilutions differed between isolates, and this difference was a measure of the serological relationship between the strains. This method was used to compare virus titers in different hosts or cultivars infected with the same strain. It is a rapid, reliable, inexpensive and sensitive technique for measuring and characterizing virus from extracts of infected plants.

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