The Effect of Decalcification on the Microspectrophotometric Determination of Dna
- 1 January 1977
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 52 (6) , 352-353
- https://doi.org/10.3109/10520297709116813
Abstract
Conventional methods of decalcification using acids depolymerize nucleic acids. This is a serious handicap for microspectrophotometric studies on the DNA content of calcified tissues. in the investigation reported here we have evaluated different decalcifying agents for this purpose. Liver, spleen, and maxillae of adult Wistar rats were exposed to the action of nitric acid, picric acid, and EDTA.Keywords
This publication has 2 references indexed in Scilit:
- CONDITIONS INFLUENCING THE INTENSITY OF THE FEULGEN REACTIONJournal of Histochemistry & Cytochemistry, 1968
- FEULGEN HYDROLYSIS: EFFECT OF ACID AND TEMPERATUREJournal of Histochemistry & Cytochemistry, 1966