Determination of lorazepam by fluorimetric and photochemical-fluorimetric methods
- 1 January 1987
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in The Analyst
- Vol. 112 (1) , 79-82
- https://doi.org/10.1039/an9871200079
Abstract
The photoisomerisation of lorazepam was studied by spectrofluorimetry and ultraviolet spectrophotometry. It was found that lorazepam leads to a strongly fluorescent photoproduct on irradiation in 0.2 M NaOH solution. The effect of pH on the fluorescence intensity of lorazepam and its photoproduct was investigated and their pKa values were calculated. A fluorimetric method with a detection limit of 16 ng ml–1 and a photochemical-fluorimetric method with a detection limit of 0.3 ng ml–1 were developed for the determination of lorazepam. The former method was applied to the determination of the drug in tablets; the latter, which is more sensitive, was used for the determination of lorazepam in serum.Keywords
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