Abstract
SummaryChromatographic studies of the auxins present in the chicory root have shown the existence of two major growth substances, a substance B, which could be tryptophane, and a substance C which is not free indolvyl-3-acetic acid, although it could be a water-soluble complex of it. The variations of endogenous auxins (as measured by the mesocotyl test) and of endogenous kinins (tested with both Osborne's and Skoog's tests) have been followed during the regeneration process of a 10-cm root piece. At the apical end, the level of the auxins drops sharply and the relative proportion of substance B to C changes, the biological activity due to B becoming greater than that due to C; meanwhile the concentration of endogenous kinins rises to reach a peak around the 3rd day, after which it decreases again, presumably due to the using up of these substances in the formation of bud primordia. At the 12th day, when buds have been regenerated at the apical end, the concentration of both auxins and kinins increases ag...