Theswi4+gene ofSchizosaccharomyces pombeencodes a homologue of mismatch repair enzymes
- 1 January 1992
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 20 (9) , 2271-2278
- https://doi.org/10.1093/nar/20.9.2271
Abstract
The swi4+ gene of Schizosaccharomyces pombe is involved in termination of copy-synthesis during mating-type switching. The gene was cloned by functional complementation of a swi4 mutant transformed with a genomic library. Determination of the nucleotide sequence revealed an open reading frame of 2979 nucleotides which is interrupted by a 68 bp long intron. The putative Swi4 protein shows homology to Duc-1 (human), Rep-3 (mouse), HexA (Streptococcus pneumoniae) and MutS (Salmonella typhimurium). The prokaryotic proteins are known as essential components involved in mismatch repair. A strain with a disrupted swi4+ gene was constructed and analysed with respect to the switching process. As in swi4 mutants duplications occur in the mating-type region of the swi4 (null) strain, reducing the efficiency of switching.Keywords
This publication has 31 references indexed in Scilit:
- CLUSTAL: a package for performing multiple sequence alignment on a microcomputerPublished by Elsevier ,2003
- Relocalization of the dorsal protein from the cytoplasm to the nucleus correlates with its functionCell, 1989
- Mechanisms of nuclear localization of the progesterone receptor: Evidence for interaction between monomersCell, 1989
- DNA MISMATCH CORRECTIONAnnual Review of Biochemistry, 1987
- Transformation of Schizosaccharomyces pombe by non-homologous, unstable integration of plasmids in the genomeCurrent Genetics, 1986
- Nuclear location signals in polyoma virus large-TCell, 1985
- A short amino acid sequence able to specify nuclear locationCell, 1984
- Sequence requirements for nuclear location of simian virus 40 large-T antigenNature, 1984
- PROTEIN-DNA RECOGNITIONAnnual Review of Biochemistry, 1984
- Plasmids carrying the yeast OMP decarboxylase structural and regulatory genes: Transcription regulation in a foreign environmentCell, 1983