Competition Between Human Plasma Albumin and Globulins for Estradiol and Sodium Estrone Sulfate

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Abstract
Equilibrium ratio determinations of estradiol and sodium estrone sulfate distributed between human red cells and solutions of human plasma albumin, fraction IV-1, or fraction IV-4 of Cohn were made at 38°C and ionic strength of 0.155. The albumin contents of the IV-1 and IV-4 fractions were analyzed by 26% sodium sulfate fractionation. When the influence of the albumin in fractions IV-1 and IV-4 was estimated on the basis of the performance of pure albumin solution, the influence of the globulins was small even on a molar basis. The red cell:albumin ratio, using a physiologic albumin concentration, was nearly the same as the endogenous ratio previously obtained for last trimester human red cells: serum. It is concluded that albumin is responsible for 90% or more of the orientation in human plasma of the estrogens studied.