Action of diphtheria toxin does not depend on the induction of large, stable pores across biological membranes
- 1 January 1990
- journal article
- conference paper
- Published by Springer Nature in The Journal of Membrane Biology
- Vol. 113 (1) , 67-74
- https://doi.org/10.1007/bf01869607
Abstract
Vero cells exposed to diphtheria toxin at pH 4.5 leak monovalent cations but not amino acids or phosphorylated metabolites; affected cells do not take up trypan blue. Monovalent cation leakage is inhibited by 1mmCd2+, but not by 1mmZn2+ or Ca2+. Cd2+ blocks calcein leakage from liposomes and closes diphtheria toxin-induced channels in lipid bilayers. It is concluded that translocation of the A fragment of diphtheria toxin across biological membranes does not depend on the formation of large stable pores, but that small Cd2+-sensitive pores may play a role.Keywords
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