The Lethal Dose of Largemouth Bass Virus in Juvenile Largemouth Bass and the Comparative Susceptibility of Striped Bass
- 1 September 1999
- journal article
- Published by Oxford University Press (OUP) in Journal of Aquatic Animal Health
- Vol. 11 (3) , 246-252
- https://doi.org/10.1577/1548-8667(1999)011<0246:tldolb>2.0.co;2
Abstract
Largemouth bass virus (LMBV) is an iridovirus that was isolated from wild adult largemouth bass Micropterus salmoides in the southeastern United States in 1994. Although originally isolated from moribund wild fish, its virulence to juvenile largemouth bass is uncertain. To help clarify this point, two LMBV titrations were made in juvenile largemouth bass. Titers of LMBV in fathead minnow cells were 104.8 and 105.8 tissue culture infectious doses—50% cytopathic endpoint (TCID50) per milliliter, respectively. Tenfold serial dilutions of LMBV employed in each cell culture titration, injected intraperitoneally (0.1 mL/fish) into largemouth bass produced calculated lethal dose—50% mortality endpoints (LD50s) of 282 (102.45) and 288 (102.46) infectious doses in two consecutive infectivity trials. Virus yield of assayed infected fish averaged 108.5 TCID50/g and 107.7 TCID50/g in viscera of moribund and dead fish in the two trials and 106.5 TCID50/g in surviving exposed fish 14 d after infection. In a se...Keywords
This publication has 11 references indexed in Scilit:
- Molecular Characterization, Sequence Analysis, and Taxonomic Position of Newly Isolated Fish IridovirusesVirology, 1997
- Iridoviruses associated with epizootic haematopoietic necrosis (EHN) in aquacultureWorld Journal of Microbiology and Biotechnology, 1997
- An Iridovirus Isolated from Wild Largemouth BassJournal of Aquatic Animal Health, 1996
- New viruses described in finfish from 1988–1992Annual Review of Fish Diseases, 1993
- Propentes of three iridovirus-like agents associated with systemic infections of fishDiseases of Aquatic Organisms, 1992
- Outbreaks of an EHNV‐like iridovirus in cultured rainbow trout, Salmo gairdneri Richardson, in AustraliaJournal of Fish Diseases, 1988
- First virus isolation from Australian fish: an iridovirus‐like pathogen from redfin perch, Perca fluviatilis L.Journal of Fish Diseases, 1986
- Asymptotically Efficient Rank Invariant Test ProceduresJournal of the Royal Statistical Society. Series A (General), 1972
- Nonparametric Estimation from Incomplete ObservationsJournal of the American Statistical Association, 1958
- Beitrag zur kollektiven Behandlung pharmakologischer ReihenversucheNaunyn-Schmiedebergs Archiv für experimentelle Pathologie und Pharmakologie, 1931