Counting Basic Sites in Oligopeptides via Gas-Phase Ion Chemistry
- 1 February 1997
- journal article
- research article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 69 (3) , 281-285
- https://doi.org/10.1021/ac961119m
Abstract
Cations derived from oligopeptides ranging from laminin fragment (5 residues) to β-lactoglobulin (162 residues) have been subjected to gas-phase ion/molecule reactions with hydroiodic acid. The sum of the ion charge state and the maximum number of molecules of hydroiodic acid that attach to the ion is equal to the total number of lysines, arginines, histidines, and N-termini consisting of a primary amine for ions derived from all 21 oligopeptides studied. These results suggest that ion/molecule reactions can provide useful information regarding oligopeptide basic site number, which might be used as a criterion for searching protein data bases instead of, or in conjunction with, use of proteolytic digestion or gas-phase ion dissociation procedures.Keywords
This publication has 27 references indexed in Scilit:
- Product Ion Charge State Determination via Ion/Ion Proton Transfer ReactionsAnalytical Chemistry, 1996
- A Direct Comparison of "First" and "Second" Gas Phase Basicities of the Octapeptide RPPGFSPFJournal of the American Chemical Society, 1995
- Ion/Molecule Reactions for Improved Effective Mass Resolution in Electrospray Mass SpectrometryAnalytical Chemistry, 1995
- Deprotonation reactions of multiply protonated ubiquitin ionsRapid Communications in Mass Spectrometry, 1994
- Proton‐transfer reactions of mass‐selected multiply charged ionsRapid Communications in Mass Spectrometry, 1994
- Probing qualitative conformation differences of multiply protonated gas-phase proteins via hydrogen/deuterium isotopic exchange with water-d2Journal of the American Chemical Society, 1992
- Charge determination of product ions formed from collision-induced dissociation of multiply protonated molecules via ion/molecule reactionsAnalytical Chemistry, 1991
- Reactions of dimethylamine with multiply charged ions of cytochrome cJournal of the American Chemical Society, 1990
- Laser desorption ionization of proteins with molecular masses exceeding 10,000 daltonsAnalytical Chemistry, 1988
- Mass spectrometric determination of the amino acid sequence of peptides and proteinsMass Spectrometry Reviews, 1987