Selective breakage of ONA alongside 5-bromodeoxyuridine nucleotide residues by high temperature hydrolysis
- 1 January 1977
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 4 (4) , 969-987
- https://doi.org/10.1093/nar/4.4.969
Abstract
The substitution of thymine mucleotides (pT) in oligodeoxynucleotides by bromouracil nucleotides (pBU) changes the properties of the oligonucleotides in two ways: (1) It alters their mobility during DEAE-Cellulose homochromatography1. (2) It substantially enhances their sensitivity to high temperature hydrolysis under mildly alkaline conditions (pH 8.9). The resultant breaks occur adjacent to pBU residues and leave terminal phosphates on the breakage products. With more extreme conditions some loss of terminal phosphates can occur. Heating at 100 degrees for 16 hr at pH 8.9 produces cleavage at about half of the pBU residues with minimal loss of terminal phosphates. The properties described here may explain the thermal sensitivity of bacteria grown in 5BU2 and may have a use in DNA sequencing technology.Keywords
This publication has 16 references indexed in Scilit:
- Differences in the incorporation of bromodeoxyuridine by human lymphoblastoid cell linesCell, 1975
- Determination of a nucleotide sequence in bacteriophage f1 DNA by primed synthesis with DNA polymeraseJournal of Molecular Biology, 1974
- Direct determination of DNA nucleotide sequences: Structure of a fragment of bacteriophage φX174 DNAJournal of Molecular Biology, 1974
- Total Substitution of Bromodeoxyuridine for Thymidine in the DNA of a Bromodeoxyuridine-Dependent Cell LineProceedings of the National Academy of Sciences, 1974
- Giemsa technique for the detection of sister chromatid exchangesChromosoma, 1974
- Use of DNA Polymerase I Primed by a Synthetic Oligonucleotide to Determine a Nucleotide Sequence in Phage f1 DNAProceedings of the National Academy of Sciences, 1973
- Determination of the Nucleotide Sequence of a Fragment of Bacteriophage ϕX 174 DNANature New Biology, 1973
- Studies on depurination of DNA by heatJournal of Molecular Biology, 1962
- Studies on Ultraviolet Irradiation of Escherichia coli Containing 5-Bromouracil in its DNAJournal of General Microbiology, 1960
- Dispersive transfer of the parental DNA molecule to the progeny of phage ∅X-174Virology, 1959