Studies on Embryonic Determination of the Harlequin-fly, Chironomus dorsalis : I. Effects of centrifugation and of its Combination with Constriction and Puncturing

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Abstract
There have been several published works on dipteran embryonic determination (Reith, 1925; Pauli, 1927; Howland et al., 1935, 1936, 1941; Cornice & Selman, 1955). These papers, however, deal primarily with the development of higher Diptera such as the house-fly and the fruit-fly. Furthermore, the eggs of such higher Dipterans are surrounded by tough and opaque chorions which often hinder detailed observations. The eggs of the harlequin-fly, on the contrary, are known to be very transparent, and as a matter of fact these eggs were extensively used during the first quarter of this century for the tracing of the germ line. In the present experiment the eggs of Chironomus dorsalis were used for two purposes: (1) to observe directly changes within the egg after various treatments, taking advantage of their transparency, and (2) to contribute to the analysis of the developmental mechanism of lower members of the Diptera.

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