PCR-Based RNA Probes: A Quick and Sensitive Method to Improve Whole Mount Embryo In Situ Hybridizations
Open Access
- 1 April 2001
- journal article
- Published by Taylor & Francis in BioTechniques
- Vol. 30 (4) , 768-774
- https://doi.org/10.2144/01304st02
Abstract
We have developed a PCR-based technique for the preparation of RNA probes that can be used for whole mount in situ hybridization on embryos. T3 or T7 RNA polymerase promoters were introduced at the...Keywords
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