SENSITIVITY IN ELECTRON MICROSCOPE AUTORADIOGRAPHY I. THE EFFECT OF RADIATION DOSE

Open Access

1 June 1972

journal article
research article
Published by SAGE Publications in Journal of Histochemistry & Cytochemistry

Vol. 20 (6) , 425-434
https://doi.org/10.1177/20.6.425

Abstract

Sensitivity in electron microscope autoradiography using Ilford L4 emulsion was shown to be affected by radiation dose (i.e., number of decays in test specimen per unit surface area). The sensitivity tended to be higher with lower doses. This dose dependence was most marked with Microdol X and least with gold latensification-Elon ascorbic acid development. Possible consequences for quantitation in electron microscope autoradiography are discussed.

Keywords

GOLD
ELECTRON MICROSCOPE
ASCORBIC
ILFORD
MICROSCOPE AUTORADIOGRAPHY
MICRODOL
LATENSIFICATION