Cell walls in Cucurbita maxima cotyledons in relation to imbibition

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Abstract
The scanning electron microscope and the freeze-etch technique were used to demonstrate the presence of corrugated cell walls in cotyledon mesophyll cells from dry squash seeds. These wavy cell walls straightened out during imbibition. In the scanning electron micrographs no wavy cell walls were observed in the smaller epidermal cells. Tissue imbibed for 2 h and then air-dried contained corrugated cell walls, whereas similar imbibed tissue dehydrated in ethanol and propylene oxide contained smooth cell walls. Thus the method of seed dehydration is important in determining the appearance of wavy cell walls. Glutaraldehyde fixation during the 2-h imbibition process did not alter the conditions under which wavy cell walls reappear.
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