Effects of Cu2+, EDTA, and Detergents on the Autoxidation of Dithiothreitol
- 1 March 1978
- journal article
- research article
- Published by Oxford University Press (OUP) in Agricultural and Biological Chemistry
- Vol. 42 (3) , 593-598
- https://doi.org/10.1080/00021369.1978.10863024
Abstract
In the absence of EDTA, the rate of autoxidation of dithiothreitol (2×10−5 m) increased with an increase of the concentration of Cu2+, and reached to the maximum at 5×10−6 m Cu2+. And then the rate declined and the autoxidation of dithiothreitol ceased at 1.6×10−5 m Cu2+. In the presence of EDTA, both the rate and the optimum concentration of Cu2+ increased. A model which involves Cu2+-EDTA or Cu2+-(dithiothreitol)2 and O2 complexes for the catalysis of the autoxidation of dithiothreitol was proposed. The cationic micelle of hexadecyltrimethylammonium bromide and the nonionic micelle of polyoxyethylene dodecyl ether also accelerated the autoxidation of dithiothreitol The microenvironmental effects on these reactions were discussed.This publication has 2 references indexed in Scilit:
- Superoxide dismutase: Improved assays and an assay applicable to acrylamide gelsAnalytical Biochemistry, 1971
- Dithiothreitol, a New Protective Reagent for SH Groups*Biochemistry, 1964