Enzymic synthesis of sym-homospermidine in Lathyrus sativus (grass pea) seedlings
- 15 August 1980
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 190 (2) , 461-464
- https://doi.org/10.1042/bj1900461
Abstract
An enzyme catalysing the synthesis of sym-homospermidine from putrescine and NAD+ with concomitant liberation of NH3 was purified 100-fold from Lathyrus sativus (grass pea) seedlings by affinity chromatography on Blue Sepharose. This thiol enzyme had an apparent mol.wt. of 75000 and exhibited Michelis-Menten kinetics with Km 3.0mM for putrescine. The same enzyme activity could also be demonstrated in the crude extracts of sandal (Santalum album) leaves, but with a specific activity 15-fold greater than that in L. sativus seedlings.This publication has 19 references indexed in Scilit:
- Occurrence of norspermidine in some species of genera Vibrio and BeneckeaBiochemical and Biophysical Research Communications, 1979
- Occurrence of sym‐homosphermidine in the Japanese newt, Cynops pyrrhogaster pyrrhogasterFEBS Letters, 1979
- Polyamine distribution in eukaryotes: occurrence of sym‐nor‐spermidine and sym‐nor‐spermine in arthropodsFEBS Letters, 1978
- Putrescine‐Sensitive (Artifactual) and Insensitive (Biosynthetic) S‐Adenosyl‐L‐Methionine Decarboxylase Activities of Lathyrus sativus SeedlingsEuropean Journal of Biochemistry, 1977
- Identification of the unusual polyamines 3,3′-diaminodipropylamine and n,n′-bis(3-aminopropyl)-1,3-propanediamine in the white shrimp Penaeus setiferusBiochemical and Biophysical Research Communications, 1977
- Thermine: A new polyamine from an extreme thermophileBiochemical and Biophysical Research Communications, 1975
- Paper chromatographic resolution of aliphatic guanidino amines, diamines and polyaminesJournal of Chromatography A, 1973
- sym-Homospermidine, a naturally occurring polyamineBiochemistry, 1971
- Tumor inhibitors. XLIII. Solapalmitine and solapalmitenine, two novel alkaloid tumor inhibitors from Solanum tripartitumThe Journal of Organic Chemistry, 1969
- The separation of aliphatic amines by paper chromatography or paper electrophoresisArchives of Biochemistry and Biophysics, 1958