Method for recovering viruses from river water solids
- 1 April 1980
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 39 (4) , 850-853
- https://doi.org/10.1128/aem.39.4.850-853.1980
Abstract
Small numbers of virions (poliovirus 1) that were adsorbed to river water solids were eluted by mixing the solids for 30 min with a 10% solution of beef extract that contained sufficient Na2HPO4 to bring the molarity of the salt to 0.05 and sufficient citric acid to bring the pH to 7. The virions were recovered by inoculating the beef extract onto cell cultures [Buffalo green monkey BGM]. With this method, 39-63% of the poliovirions that were adsorbed onto the river solids were recovered.This publication has 3 references indexed in Scilit:
- Organic Flocculation: an Efficient Second-Step Concentration Method for the Detection of Viruses in Tap WaterApplied and Environmental Microbiology, 1976
- BGM, a continuous cell line more sensitive than primary rhesus and African green kidney cells for the recovery of viruses from water.1974
- Recovery of small quantities of viruses from clean waters on cellulose nitrate membrane filters.1971