Purification of Hog Renin. Properties of Purified Hog Renin
- 1 January 1972
- journal article
- research article
- Published by Taylor & Francis in Scandinavian Journal of Clinical and Laboratory Investigation
- Vol. 29 (1) , 51-58
- https://doi.org/10.3109/00365517209081055
Abstract
Hog kidneyswere minced, lyophilized, extracted with acetone/water, and the extract was gel-filtrated. The partly purified renin was used for analytical ultracentrifugation and for electrofocusing. By these methods the molecular weight of renin was determined to be 4 × 104g/M. Four distinct fractions of enzymatically and immunologically identical renin with pI's between pH 4.75–5.10 were shown. Activation of renin by treatment with acid at pH 3.7 was investigated by means of inhibitors and activators of proteolytic enzymes.Keywords
This publication has 11 references indexed in Scilit:
- Spectrophotometric determination of microgram quantities of protein without nucleic acid interferenceAnalytical Biochemistry, 1968
- Chemical Assay of Phospholipid Renin Preinhibitor in Canine and Human BloodCirculation Research, 1967
- Purification of pig reninBiochemical Journal, 1966
- Molecular Weight of Renin Determined by Sephadex G-200 Gelfiltration.Acta Chemica Scandinavica, 1966
- Estimation of the molecular weights of proteins by Sephadex gel-filtrationBiochemical Journal, 1964
- Molecular Weight of Renin Determined by Sephadex Gel-filtration.Acta Chemica Scandinavica, 1964
- Removal of angiotensinase from a preparation of reninArchives of Biochemistry and Biophysics, 1962
- The active site of esterasesJournal of Cellular and Comparative Physiology, 1959
- A simple method for the extraction and partial purification of reninArchives of Biochemistry and Biophysics, 1954
- Isolation and purification of hog reninArchives of Biochemistry and Biophysics, 1953