Estimation of the light distribution between photosystems I and II in intact wheat leaves by fluorescence and photoacoustic measurements

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Abstract
Usisng intact leaves, the extent of the decrease in chlorophyll a fluorescenece caused by the addition of continuous 710 nm light superimposed on modulated (20 Hz) 550 nm light was used to determine the distribution of this absorbed light between photosystems I (α) and II (β). The Fo and Fm levels, which defined the total variable fluorescenece, were taken as equal to those obtained with excess 710 nm light and with saturating blue-green light, respectively. An analogous procedure was used with a photoacoustic detector, saturating white light defining a base line for oxygen yield, the levels with an without 710 nm light being used to define β and α respectively. The two methods gave similar values for the distribution of light between the two photosystems for the experimental conditions used, β averaging 0.55 for a range of Triticum genotypes and Brachypodium sylvaticum grown in high or low light.

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