Multiphoton Microscopy

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Abstract
Multiphoton microscopy (MM) was not commonly known until Goppert-Mayer1 introduced the theoretical prediction of two-photon absorption in the 1930s. MM technology became popular and was met with interest among biomedical scientists after the introduction of high-speed lasers and more importantly because of the contribution made by Watt Webb’s group from Cornell University.2 It’s also important to mention that the commercialization of this multiphoton technology by Bio-Rad Laboratories has created much awareness of the usage of this technology in biomedical imaging. Currently, there are many leading light microscopy centers and laboratories involved in developing new MM technology for implementation in various biomedical applications. Since 1990, many papers have been published in peer-reviewed journals and presented at conferences, and books have been published focusing specifically on MM technology and its applications in the biomedical sciences.3 4 5 6 7 8 We believe that the future is headed toward easier-to-use equipment, lower cost, and higher sensitivity, which will allow the user greater flexibility in imaging multiple fluorochromes simultaneously, while collecting images from a single cell to tissue.