Bacillus subtilis DNA gyrase: purification of subunits and reconstitution of supercoiling activity
- 1 July 1982
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 151 (1) , 524-527
- https://doi.org/10.1128/jb.151.1.524-527.1982
Abstract
DNA gyrase from B. subtilis 168 was purified by affinity chromatography on novobiocin-Sepharose and shown to consist of 2 subunits, A and B, with MW of 100,000 and 85,000, respectively. The B subunit, which contains novobiocin-sensitive ATPase activity, could complement the gyrA protein of Escherichia coli. No complementation was detected between the A subunit and the E. coli gyrB protein.This publication has 13 references indexed in Scilit:
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