Hemoglobin Repletion in Anemic Rats Fed a New Bean Cultivar (Phaseolus vulgaris L.) as Source of Iron.

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Abstract
Utilization of iron from a new cultivar of dry bean (Carioca 80 SH) was estimated using anemic rat by two methods: the slope ratio and the hemoglobin regeneration methods. The bean was incorporated into the diet in the form of whole bean flour (WBF) 7.45 mg Fe/100 g or as partially decorticated bean flour (DBF), 8.57 mg Fe/100 g. Hemoglobin concentration in the rats' blood at the beginning of the experiment averaged 13.79+/-0.33 g Hb/dl; at the end of the depletion period it ranged from 5.62+/-0.38 to 6.30+/-0.39 g Hb/dl blood, difference which was not statistically significant (p less-than-or-equal-to 0.05). The bean iron relative biological value (RBV, relative to FeSO4=100) evaluated by the slope ratio method, was 74% for the WBF and 77% for the DBF. The hemoglobin regeneration efficiency was 60.4+/-6.5, 50.8+/-3.2, and 52.7+/-5.7%, respectively, for the iron sulfate (reference source), the WBF, and the DBF.