Solvent Effects on the Precision of Sample Application in Quantitative Thin-Layer Chromatography
- 1 January 1980
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 3 (6) , 833-839
- https://doi.org/10.1080/01483918008060194
Abstract
The use of non-polar solvents for sample application in quantitative thin-layer chromatography (TLC) was shown to have a marked effect on the precision of the analytical technique when polar compounds were applied to silica gel plates. Increasing the polarity of the application solvent allowed competitive binding of the solvent molecules to silica, thereby deactivating the silica at the origin. Studies showed that the coefficient of variation for TLC assays were substantially reduced by altering the polarity of the application solvent.Keywords
This publication has 2 references indexed in Scilit:
- Solute-solvent interactions on the surface of silica gel. IIJournal of Chromatography A, 1979
- The scope of thin-layer chromatographyJournal of Chromatography A, 1973