Adenovirus DNA replication in vitro is stimulated by RNA from uninfected HeLa cells
- 4 June 1984
- journal article
- Published by Wiley in FEBS Letters
- Vol. 171 (1) , 5-8
- https://doi.org/10.1016/0014-5793(84)80449-4
Abstract
Adenovirus DNA replication was studied in a partially reconstituted system consisting of purified viral proteins (DNA‐binding protein, precursor terminal protein and Ad DNA polymerase) and a nuclear extract from uninfected HeLa cells. Optimal DNA replication required the presence of a heat‐stable, ribonuclease‐sensitive fraction from the cytosol of uninfected cells. This fraction stimulated the initiation about 3‐fold and the replication of origin fragments 5–10‐fold. Sedimentation analysis indicated the presence of a fast‐sedimenting and a slow‐sedimenting component which complemented each other. At least part of the stimulation was caused by low‐molecular‐mass RNA.Keywords
This publication has 13 references indexed in Scilit:
- Crystallization of a fragment of the adenovirus DNA binding proteinJournal of Molecular Biology, 1984
- The replication of adenovirus DNA with purified proteinsCell, 1983
- Biochemical complementation with RNA in the Xenopus oocyte: A small rna is required for the generation of 3′ histone mRNA terminiCell, 1983
- Adenovirus DNA replication in vitro: synthesis of full-length DNA with purified proteins.Proceedings of the National Academy of Sciences, 1983
- The U1 small nuclear RNA-protein complex selectively binds a 5′ splice site in vitroCell, 1983
- Specific binding of the adenovirus terminal protein precursor-DNA polymerase complex to the origin of DNA replicationNucleic Acids Research, 1983
- Isolation of Proteins Involved in the Replication of Adenoviral DNA In vitroPublished by Cold Spring Harbor Laboratory ,1983
- Adenovirus DNA replication in vitro: identification of a host factor that stimulates synthesis of the preterminal protein-dCMP complex.Proceedings of the National Academy of Sciences, 1982
- Eukaryotic DNA Replication: Viral and Plasmid Model SystemsAnnual Review of Biochemistry, 1982
- Adenovirus DNA replication in vitro.Proceedings of the National Academy of Sciences, 1979