Housekeeping genes for quantitative expression studies in the three-spined stickleback Gasterosteus aculeatus
Open Access
- 29 January 2008
- journal article
- Published by Springer Nature in BMC Molecular Biology
- Vol. 9 (1) , 18
- https://doi.org/10.1186/1471-2199-9-18
Abstract
During the last years the quantification of immune response under immunological challenges, e.g. parasitation, has been a major focus of research. In this context, the expression of immune response genes in teleost fish has been surveyed for scientific and commercial purposes. Despite the fact that it was shown in teleostei and other taxa that the gene for beta-actin is not the most stably expressed housekeeping gene (HKG), depending on the tissue and experimental treatment, the gene has been used as a reference gene in such studies. In the three-spined stickleback, Gasterosteus aculeatus, other HKG than the one for beta-actin have not been established so far.Keywords
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