Demonstration of Conjugated Dopamine in Monkey CSF by Gas Chromatography-Mass Spectrometry
- 1 September 1982
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 39 (3) , 726-728
- https://doi.org/10.1111/j.1471-4159.1982.tb07952.x
Abstract
A method for measuring unconjugated and conjugated dopamine in body tissues and fluids is described. Conjugated dopamine was hydrolyzed in acid to unconjugated dopamine, separated from the sample matrix by alumina chromatography, and assayed by gas chromatography-mass spectrometry. Conjugated dopamine was detected in greater concentrations than unconjugated dopamine in CSF taken from lateral ventricle or thecal sac of the Rhesus monkey. Haloperidol administration did not increase the levels of conjugated dopamine in lumbar CSF.Keywords
This publication has 9 references indexed in Scilit:
- Free and conjugated dopamine in human ventricular fluidBrain Research, 1981
- Presence of Conjugated Catecholamines in Rat Brain: A New Method of Analysis of Catecholamine SulfatesJournal of Neurochemistry, 1981
- Contribution of plasma homovanillic acid (HVA) to urine and cerebrospinal gluid HVA in the monkey and its pharmacokinetic dispositionLife Sciences, 1978
- Effects of haloperidol and probenecid on plasma and CSF dopamine metabolites in the rhesus monkey (Macacca Mulatta)Life Sciences, 1978
- Dihydroxyphenylacetic acid conjugate: Natural occurrence and demonstration of probenecid-induced accumulation in rat striatum olfactory tubercles and frontal cortexEuropean Journal of Pharmacology, 1977
- Conjugated 3,4 dihydroxy phenyl acetic acid (DOPAC) in human and monkey cerebrospinal fluid and rat brain and the effects of probenecid treatmentLife Sciences, 1976
- Simultaneous measurement of endogenous and deuterium-labeled tracer variants of choline and acetylcholine in subpicomole quantities by gas chromatography/mass spectrometryAnalytical Biochemistry, 1973
- Studies on the sulphation of 3,4-dihydroxyphenylethylamine (dopamine) and related compounds by rat tissuesBiochemical Journal, 1973
- A rapid, simplified procedure for simultaneous assay of norepinephrine, dopamine, and 5-hydroxytryptamine from discrete brain areasAnalytical Biochemistry, 1971