INFECTION OF THE ONE-DAY OLD FERTILE HEN'S EGG WITH RABIES VIRUS

Abstract
Thus far, little knowledge is available concerning the use in virus studies of fertile hen's eggs inoculated early in embryonic development. Weiss and Huang (1954) noted inclusion bodies in 15-hour-old blastodermal cells infected with meningopneumonitis virus in tissue culture. Scott and Yoshino (unpublished data cited in Yoshino, 1956) found that 1-day egg's blastoderm could support herpes simplex virus multiplication in tissue culture. Hamburger and Habel (1947) infected 2-day old eggs with influenza and mumps viruses and found embryonic anomalies in a high proportion. Shear et al. (1955) demonstrated that this teratogenic effect by influenza virus could be neutralized by antiserum. A similar teratogenic effect was noted with Newcastle disease virus (Williamson et al., 1953) . Recently, a simple technique for infection of the 1-day egg was described by Yoshino (1956), and it was shown that this host was highly susceptible to herpes virus. When the same technique of inoculation was used with rabies virus, rapid multiplication occurred together with apparently normal embryonic development for a few days. Further studies revealed that rabies virus in high titer could be obtained from 1-day eggs.
Keywords

This publication has 1 reference indexed in Scilit: