Catecholamine histofluorescence using cryostat sectioning and glyoxylic acid in unperfused frozen brain: a detailed description of the technique

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Abstract
This paper presents a technique for glyoxylic acid-induced monoamine histofluorescence in 2% glyoxylic acid solution, warm-air dried and gassed at 100°C. Intense, well-localized catecholamine fluorescence is produced and all known catecholamine-containing structures are demonstrated. The fluorescence obtained by this method was evaluated by a variety of agents and was shown to be catecholaminergic in origin. In contrast to the Vibratome—glyoxylic acid technique, this procedure reliably produces thin, whole-brain sections of even thickness and allows protracted use of the tissue block. Because unperfused tissue is used, the technique can be applied to a broad variety of material, such as post-mortem tissue or invertebrate preparations. Alternate sections can be prepared for a variety of techniques requiring unperfused tissue (e.g., enzymatic localization, chemical assay, anatomical techniques). The reasons for choosing each of the parameters in the technique are discussed.

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