ON THE RIBONUCLEIC ACID SYNTHESIZED IN A CELL-FREE SYSTEM OF ESCHERICHIA COLI
- 1 March 1962
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 48 (3) , 425-430
- https://doi.org/10.1073/pnas.48.3.425
Abstract
Ribonucleic acid (RNA) can be continuously synthesized in the 105,000 X g supernatant of Escherichia coli in the presence of 4 ribonucleoside triphosphates, deoxyribonucleic acid (DNA), an energy generation system and salmine-sulfate. The RNA is well protected from degradation by salmine-sulfate. The addition of salmine makes it possible to prepare the "undegraded" RNA by the phenol method. The synthesized RNA behaves essentially like the "undegraded" messenger-RNA. These observations, together with the identity of the base ratios (which are equivalent to those of DNA) of these RNAs support the view that the RNA synthesized in the cell free system is the messenger-RNA.Keywords
This publication has 13 references indexed in Scilit:
- THE SELECTIVE SYNTHESIS OF INFORMATIONAL RNA IN BACTERIAProceedings of the National Academy of Sciences, 1961
- THE ENZYMATIC SYNTHESIS OF RNA: COMPLEMENTARY INTERACTION WITH DNAProceedings of the National Academy of Sciences, 1961
- FURTHER STUDIES ON DEOXYRIBONUCLEIC ACID-DEPENDENT ENZYMATIC SYNTHESIS OF RIBONUCLEIC ACIDProceedings of the National Academy of Sciences, 1961
- ON THE PARTICIPATION OF DNA IN RNA BIOSYNTHESISProceedings of the National Academy of Sciences, 1961
- DEOXYRIBONUCLEIC ACID-DEPENDENT INCORPORATION OF NUCLEOTIDES FROM NUCLEOSIDE TRIPHOSPHATES INTO RIBONUCLEIC ACIDProceedings of the National Academy of Sciences, 1961
- Unstable Ribonucleic Acid Revealed by Pulse Labelling of Escherichia ColiNature, 1961
- Enzymatic synthes is of RNABiochemical and Biophysical Research Communications, 1960
- A fractionating column for analysis of nucleic acidsAnalytical Biochemistry, 1960
- High-Resolution Density Gradient Sedimentation AnalysisScience, 1960
- Amino acid analog incorporation into bacterial proteinsBiochimica et Biophysica Acta, 1959