Differential Protein Synthesis in Candida albicans during Blastospore Formation at 24.5 C and during Germ Tube Formation at 37 C

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Abstract
To identify proteins synthesized during blastospore to germ tube transformation in C. albicans, membrane and cytoplasmic protein fractions labeled with 14C were analyzed by SDS-PAGE [sodium dodecylsulfate polyacrylamide gel electrophoresis] and autoradiography. Four cytoplasmic proteins were detected in pulse-labeled lysates prepared from cells forming blastospores and germ tubes at 37.degree. C, but not in pulse-labeled lysates prepared from cells forming blastospores at 24.5.degree. C. Three proteins detectable in 24.5.degree. C lysates were diminished in 37.degree. C lysates.

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