A new catheter system for cannulation of thoracic duct of the rat

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Abstract
A new catheter system for cannulation of thoracic duct of the rat is described. Lymph from cannulated thoracic duct flows via perforations in the catheter into a mixing chamber which en-sleeves the perforated portion of the catheter. A constant micro-infusion pump delivers dilute heparin solution via a separate catheter into the chamber at an extremely slow rate of approximately 1 ml/24 hr. Thus, lymph flows freely in the catheter system into a collection vessel without any back flow of heparin into the animal. The catheter system is designed to prevent coagulation of lymph in vitro. It has proved effective in maintaining good lymph flow (30-100 ml/day) for periods up to 2 weeks. The preparation should prove useful in studies of absorption and chylous transport of lipids in the rat.

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