Magnetic bead capture of cDNAs from double-stranded plasmid cDNA libraries
Open Access
- 1 August 1997
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 25 (15) , 3183-3185
- https://doi.org/10.1093/nar/25.15.3183
Abstract
We have developed a cDNA library screening method which allows the simultaneous screening of >1012 double-stranded plasmid cDNA molecules with minimal a priori sequence knowledge. A biotinylated, genespecific oligonucleotide probe along with abutting ‘blocking’ oligos is hybridized to the plasmid cDNA library and the target plasmid retrieved with paramagnetic streptavidin beads and transformed into Escherichia coli. Multiple rounds of enrichment with a target plasmid represented at 0.002–0.0001% resulted in over one-third positive clones. Our method will be useful for isolating even the rarest cDNAs starting from ESTs, isolated exons or homologous sequence information.Keywords
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