SECRETION DEFECT IN PLATELETS STORED AT 4-DEGREES-C

  • 1 January 1982
    • journal article
    • research article
    • Vol. 47  (3) , 221-225
Abstract
To understand the functional changes induced by storage, the adenine nucleotides of [human] platelets stored for 72 h at 22.degree. and 4.degree. C were examined. Ten platelet concentrates (PC) were stored at each temperature, with 5 in each group having a final volume of 50 and 30 ml. The total ATP and ADP content of platelets decreased following storage in all 4 groups of PC, with the decrease being greater in the PC stored at 22.degree. C than those at 4.degree. C. The mean thrombin secretable ATP + ADP content of platelets from PC stored at 22.degree. and 4.degree. C were 29.7 and 19.7% of the total content, respectively (P < 0.001). Cold-stored platelets had a higher total content of ATP + ADP but secreted distinctly lesser amounts than 22.degree. C-stored platelets. Labeling of the metabolic pool adneylates with 14C-adenine revealed a greater decrease in the adenylate energy charge of the platelets stored at 4.degree. C. The secretion defect demonstrated in cold-stored platelets may be related to the inability of these platelets to maintain ATP homeostasis.