Immunogenic proteins of Leishmania major during mouse infection

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Abstract
Mice of different strains (C3H/He, A/Jax, DBA/2, BALB/c and CBA/Ca) were infected intracutaneously with L. major. Antibody production was analyzed on the antigens of the promastigote form of the parasite, previously separated by SDS[sodium dodecyl sulfate]-polyacrylamide gel electrophoresis and transferred to nitrocellulose sheets. The kinetics of IgG antibody production were determined and the major immunogenic proteins of the parasite were identified for each strain of mouse. Antibody directed against parasite proteins appeared in the blood of all mouse strains tested soon after the 1st wk of infection and rose steadily for 5-12 wk. Qualitative individual variation within a given strain was commonly observed in the response to the various antigens. Each mouse strain synthesized IgG directed against a somewhat different spectrum of parasite proteins. Homologies in antigen recognition by pooled sera of 5 mice ranged from 54% to 79% from one mouse strain to another. Hybrid mice from a BALB/c .times. CBA cross (F1 generation) recognized essentially all antigens recognized by the 2 parents. Proteins of L. major with apparent MW of 19 kD [kilodaltons] 60 kD, 65 kD and > 100 kD were immunogenic in all strains of mice tested.

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