Identification and Characterization of the Penicillin-Binding Protein 2a of Streptococcus pneumoniae and Its Possible Role in Resistance to β-Lactam Antibiotics
Open Access
- 1 June 2000
- journal article
- Published by American Society for Microbiology in Antimicrobial Agents and Chemotherapy
- Vol. 44 (6) , 259-261
- https://doi.org/10.1128/aac.44.6.1745-1748.2000
Abstract
To further understand the role of penicillin-binding protein 2a (PBP 2a) of Streptococcus pneumoniae in penicillin resistance, we confirmed the identity of the protein as PBP 2a. The PBP 2a protein migrated electrophoretically to a position corresponding to that of PBP 2x, PBP 2a, and PBP 2b of S. pneumoniae and was absent in a pbp2a insertional mutant of S. pneumoniae . We found that the affinities of PBP 2a for penicillins were lower than for cephalosporins and a carbapenem. When compared with other S. pneumoniae PBPs, PBP 2a exhibited lower affinities for β-lactam antibiotics, especially penicillins. Therefore, PBP 2a is a low-affinity PBP for β-lactam antibiotics in S. pneumoniae.Keywords
This publication has 27 references indexed in Scilit:
- X-ray structure of Streptococcus pneumoniae PBP2x, a primary penicillin target enzymeNature Structural & Molecular Biology, 1996
- Penicillin-Binding Proteins as Resistance Determinants in Clinical Isolates ofStreptococcus pneumoniaeMicrobial Drug Resistance, 1996
- Genetics of high level penicillin resistance in clinical isolates ofStreptococcus pneumoniaeFEMS Microbiology Letters, 1995
- β-Lactam Antibiotic Resistance in Gram-Positive Bacterial Pathogens of the Upper Respiratory Tract: A Brief Overview of MechanismsMicrobial Drug Resistance, 1995
- Penicillin binding protein 2x as a major contributor to intrinsic β‐lactam resistance of Streptococcus pneumoniaeFEBS Letters, 1993
- Deletion analysis of the essentiality of penicillin-binding proteins 1A, 2B and 2X ofStreptococcus pneumoniaeFEMS Microbiology Letters, 1993
- Interspecies recombinational events during the evolution of altered PBP 2x genes in penicillin‐resistant clinical isolates of Streptococcus pneumoniaeMolecular Microbiology, 1991
- Nucleotide sequences of the pbpX genes encoding the penicillin‐binding proteins 2x from Streptococcus pneumoniae R6 and a cefotaxime‐resistant mutant, C506Molecular Microbiology, 1989
- Extensive re‐modelling of the transpeptidase domain of penicillin‐binding protein 2B of a penicillin‐resistant South African isolate of Streptococcus pneumoniaeMolecular Microbiology, 1989
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970