Transient Thermal Tolerance: Cell Killing and Polymerase Activities

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Abstract
Chinese hamster ovary [CHO] cells heated continuously at 42.2.degree. C became transiently thermal tolerant between 4 and 15 h of heating. During this interval, thermal tolerance was evident by a plateau in the response for both cell killing and cellular DNA polymerase-.alpha. and -.beta. activities. As heating continued beyond 15 h, both cell killing and reduction in polymerase-.beta. activity began to occur again. About 5 h later, after 20 h of heating, the activity of polymerase-.alpha. also began to decrease, but at a rate about 1/3 that observed for polymerase-.beta.. As reported previously, there is a positive correlation between hyperthermic-induced cell killing and hyperthermic reduction in cellular polymerase-.beta. activity. The correlation is only temporal, and additional studies are needed to determine if cell death is indeed related to a certain reduction in polymerase-.beta. activity.

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