GAS CHROMATOGRAPHIC ANALYSIS OF THE UNCONJUGATED, NEUTRAL NON-POLAR STEROIDS OF HUMAN PLACENTA

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Abstract
SUMMARY: Normal human term placentae have been examined for neutral non-polar steroids by gas chromatography. Preliminary examination of a number of neutral extracts showed a consistent pattern of chromatographic peaks on both non-selective (SE-30) and selective (QF-1) stationary phases. The extracts were further fractionated by thin-layer chromatography and reexamined in detail by acetate formation, adsorption and partition thin-layer chromatography, and gas chromatography. The major steroids identified were progesterone, 3β-hydroxy-pregn-5-en-20-one, 20α-hydroxy-pregn-4-en-3-one and 3β-hydroxy-5α-pregnan-20-one; minor amounts of 3α-hydroxy-5β-pregnan-20-one and 5β-pregnane-3α,20α-diol were also identified.