A reappraisal of the enzyme lipase for removing drying-oil stains on paper

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Abstract
The removal of oil stains from paper especially of drying-oils such as linseed oil, is a challenge for the conservator. The enzyme lipase is capable of causing the degradation of fats and oils based on triacylglyceride structures. The existing literature gives a confusing picture of conditions under which lipase can successfully be used in paper conservation. A series of tests on unaged, artificially aged and naturally aged test samples of linseed-oil stains on various papers were made. The results indicate that the application of lipases under the conventional application conditions exhibit a very low effectiveness, if any. The rule of buffer, wetting agent and pH is also investigated. In the light of these results Tris buffer and wetting agents are in most cases responsible for improvements to colour, bleachability and wettability of the linseed-oil stains.