CHARACTERIZATION OF STIMULATORY ACTIVITY FOR HUMAN PLURIPOTENT STEM-CELLS (CFUGEMM)

  • 1 January 1983
    • journal article
    • research article
    • Vol. 11  (2) , 154-161
Abstract
A medium conditioned by leukocytes in the presence of phytohemagglutinin (PHA-LCM) promotes the growth of human multilineage hemopoietic progenitors CFUGEMM [granulocytic, erythrocytic, monocytic and megakaryocytic colony-forming units] which form mixed hemopoietic colonies in culture containing granulocytes, erythroblasts, megakaryocytes, macrophages and mononuclear cells with T-cell antigens. In addition, PHA-LCM supports the growth of erythroid bursts (BFUE) and granulocytic colonies (CFUC). Stimulatory activities of the OHA-LCM were characterized by gel filtration using Sephacryl S 300. The eluted growth promoting activities were rechromatographed on Sepharose 4 B covalently linked with Lentil Lectin. The stimulatory activities for noncommitted precursors CFUGEMM and committed progenitors BFUE and CFUC were detected in the untrained material, i.e., the activities revealed no affinity to Lentil-Lectin. The apparent MW of these stimulatory molecules ranged from 35,000 to 45,000, as determined by SDS [sodium dodecyl sulfate] polyacrylamide gel electrophoresis (PAGE).