Double-strand cleavage at a two-base deletion mismatch in a DNA heteroduplex by nuclease S1
- 1 August 1985
- journal article
- research article
- Published by Portland Press Ltd. in Bioscience Reports
- Vol. 5 (8) , 627-632
- https://doi.org/10.1007/bf01116993
Abstract
A two-base deletion mismatch was generated in a DNA heteroduplex by hybridization of two linear plasmid DNA molecules differing only by the presence of a two-base deletion in one of them. The heteroduplex was shown to be sensitive to double-strand cleavage by nuclease S1, thus demonstrating the potential value of single-stranded probes for the detection of polymorphisms in genomic DNA due to very small deletions.Keywords
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