Gas Chromatographic Determination of Nicotine in Environmental Tobacco Smoke: Collaborative Study
- 1 November 1989
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of AOAC INTERNATIONAL
- Vol. 72 (6) , 1002-1006
- https://doi.org/10.1093/jaoac/72.6.1002
Abstract
A gas chromatographic method for determination of vapor phase nicotine in environmental tobacco smoke (ETS) was collaboratively studied by 6 laboratories. Nicotine is desorbed from XAD-4 sample tubes with ethyl acetate containing triethylamine and determined by gas chromatography with nitrogen-selective detection. Each collaborator received blind duplicate samples at each of 6 nicotine concentrations. Three concentrations were generated by spiking XAD-4 tubes with known amounts of nicotine; the remaining 3 concentrations were ETS samples obtained in a carefully controlled environmental chamber containing sidestream and exhaled mainstream smoke from 1R4F Kentucky reference cigarettes. Repeatability and reproducibility relative standard deviations ranged from 4.4 to 11.1% and from 7.0 to 11.1%, respectively, for nicotine concentrations evaluated (up to 6 ng/cu m). The method has been adopted official first action.This publication has 1 reference indexed in Scilit:
- Estimation of personal exposure to tobacco smoke with a newly developed nicotine personal monitorEnvironmental Research, 1984