Purification and Identification of the Fruiting-Inducing Substances inCoprinus macrorhizus
- 1 March 1973
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 113 (3) , 1240-8
- https://doi.org/10.1128/jb.113.3.1240-1248.1973
Abstract
Substances which are effective in inducing fruiting bodies in monokaryotic mycelia of thefis+strain ofCoprinus macrorhizuswere purified and characterized. The active components of fruiting-inducing substances were identified as adenosine-3′-monophosphate, adenosine 3′,5′-cyclic monophosphate (cyclic AMP), and a protein which is bound with the cyclic AMP. Cyclic AMP was synthesized from adenine within mycelia of the mutant strains which form monokaryotic fruiting bodies without the addition of fruiting-inducing substances, but not in those of the strains which do not form monokaryotic fruiting bodies. The proteins which bind with cyclic AMP were detected in crude extracts of mycelia of those strains which form monokaryotic fruiting bodies and of the dikaryon, but not in those of the strains which do not form monokaryotic fruiting bodies.Keywords
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