ADENOSINE 5′-TRIPHOSPHATE-ARGININE PHOSPHOTRANSFERASE FROM LOBSTER MUSCLE: PURIFICATION AND PROPERTIES
- 1 March 1965
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 94 (3) , 536-544
- https://doi.org/10.1042/bj0940536
Abstract
A simple chromatographic method is described for the purification of arginine kinase from lobster (Homarus vulgar is) muscle. Some physical properties and the effects on enzyme activity of ionic strength, pH, buffer salts, metal ions and substrates are reported. The kinetic parameters, evaluated by variation of the concentration of one of the substrates, are dependent on the concentration of the other substrate. The properties of the enzyme are discussed in relation to previous findings about phosphagen phospho-transferases.Keywords
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