ALTERATION OF HEXOSAMINIDASE ISOZYMES IN HUMAN RENAL-CARCINOMA

  • 1 January 1979
    • journal article
    • research article
    • Vol. 39  (5) , 1829-1834
Abstract
The activity and isozyme patterns of hexosaminidase [EC 3.2.1.30] in human renal carcinoma were studied in comparison with those of normal kidney. Hexosaminidase in extracts from normal kidney and renal carcinoma tissue could be separated into 2 major forms [hexosaminidase A (Hex A) and hexosaminidase B (Hex B)] by Cellogel electrophoresis or by diethylaminoethyl cellulose column chromatography. All of 10 renal carcinoma tissues showed a low activity ratio of Hex A to Hex B, as compared with the ratio in normal kidney. The ratio in renal carcinoma tissue was between 0.61-2.21 (mean, 1.30), while that in normal kidney was between 2.50 and 4.52 (mean, 3.46). Hexosaminidase activity and the ratio of Hex A to Hex B in renal carcinoma tissue were independent of the cell type and the differentiation grade of carcinoma tissue. Hex A and Hex B of renal carcinoma tissue differed from each other in physicochemical properties such as pH dependence of enzyme activity, thermostability and Kms for 2 synthetic substrates, but each isozyme maintained its same physicochemical properties whether from normal or from carcinoma tissue. The isozyme patterns of cultred renal carcinoma cells and placenta were similar to those of the carcinoma tissue. Hexosaminidase isozymes in renal carcinoma tissue apparently express at least oncoplacental patterns.

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