Experimental Allergic Encephalomyelitis after the Excision of the Injection Site of Antigen-Adjuvant Emulsion

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Abstract
It has been repeatedly shown that when certain antigenic material dissolved or suspended in physiological salt solution is injected into the subcutaneous tissue of animals, the surgical removal of the site of injection within a few minutes after injection does not prevent antibody formation (1). These observations are readily explained by the prompt dissemination of injected material. It was demonstrated by Cannon and Burt (2) that when Staphylococcus aureus or Serratia marcescens are injected into tissue of the ear of the rabbit, these microorganisms can be cultured from the blood, spleen, and liver even if the ear is amputated within a few minutes after injection. Furthermore, McMaster and Kidd (3) have shown that an intracutaneous injection introduces material very promptly into regional lymph nodes. When antigens incorporated in water-in-paraffin oil emulsion with or without mycobacteria are injected into the tissues, the immune response is abundant and long sustained.