Citrate synthase of Escherichia coli
- 5 April 1983
- journal article
- Published by Wiley in FEBS Letters
- Vol. 154 (1) , 51-54
- https://doi.org/10.1016/0014-5793(83)80873-4
Abstract
Guest [J. Gen. Microbiol. (1981) 124, 17–23] has cloned the citrate synthase gene from E. coli into ColE1 plasmids. This plasmid‐coded enzyme has now been purified and characterised and shown to be indistinguishable from the chromosome‐coded enzyme in molecular, catalytic and regulatory properties. The plasmid copy number has been raised to > 200/cell by treatment with chloramphenicol and the effects of this increase on the intracellular level of citrate synthase have been investigated.Keywords
This publication has 15 references indexed in Scilit:
- Unity and Diversity in Some Bacterial Citric Acid-Cycle EnzymesPublished by Elsevier ,1981
- Studies on a Mutant Form of Escherichia coli Citrate Synthase Desensitised to Allosteric EffectorsEuropean Journal of Biochemistry, 1979
- Crystal Structure Analysis of the Tetragonal Crystal Form and Preliminary Molecular Model of Pig‐Heart Citrate SynthaseEuropean Journal of Biochemistry, 1979
- Citrate SynthaseCurrent Topics in Cellular Regulation, 1976
- Quaternary structure of citrate synthase from Escherichia coli K 12Biochemistry, 1975
- Nature of Col E 1 Plasmid Replication in Escherichia coli in the Presence of ChloramphenicolJournal of Bacteriology, 1972
- Regulation of citrate synthase activity by α‐ketoglutarate. Metabolic and taxonomic significanceFEBS Letters, 1969
- Regulation of Citrate Synthase and Microbial TaxonomyNature, 1968
- Allosteric regulation of the activity of citrate synthetase of Escherichiacoli by α-ketoglutarateBiochemical and Biophysical Research Communications, 1967
- Reduced Nicotinamide-Adenine Dinucleotide as an Allosteric Effector of Citrate-Synthase Activity in Escherichia coliBiochemical Journal, 1966