BIOGENESIS OF CHLOROPLAST MEMBRANES
Open Access
- 1 December 1967
- journal article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 35 (3) , 553-584
- https://doi.org/10.1083/jcb.35.3.553
Abstract
Dark-grown cells of the y-1 mutant of Chlamydomonas reinhardi contain a partially differentiated plastid lacking the photosynthetic lamellar system. When exposed to the light, a rapid synthesis of photosynthetic membranes occurs accompanied by synthesis of chlorophyll, lipids, and protein and extensive degradation of the starch reserve. The process is continuously dependent on illumination and is completed within 6-8 hr in the absence of cell division. Photosynthetic activity (O(2) evolution, Hill reaction, NADP photo-reduction, and cytochrome f photooxidation) parallels the synthesis of pigment and membrane formation. During the greening process, only slight changes occur in the levels of soluble enzymes associated with the photosynthetic process (RuDP-carboxylase, NADP-linked G-3-P dehydrogenase, alkaline FDPase (pH 8)) as compared with the dark control. Also cytochrome f concentration remains almost constant during the greening process. The kinetics of the synthesis of chlorophyll, formation of photosynthetic membranes, and the restoration of photosynthetic activity suggest that the membranes are assembled from their constituents in a single-step process.Keywords
This publication has 26 references indexed in Scilit:
- BIOGENESIS OF CHLOROPLAST MEMBRANESThe Journal of cell biology, 1967
- Regulation of haemoglobin synthesis at the polysome levelJournal of Molecular Biology, 1966
- Fluorescence Changes During Chlorophyll Formation in Euglena gracilis (and Other Organisms) and an Estimate of Lamellar Area as a Function of AgeThe Journal of Protozoology, 1965
- The protein-synthesizing systems from the cytoplasm and the chloroplasts of Euglena gracilisJournal of Molecular Biology, 1964
- Lipid Metabolism of Euglena gracilisJournal of Biological Chemistry, 1964
- Lipid Alterations in Euglena gracilis Cells During Light-induced Greening*Biochemistry, 1964
- CHLOROPLAST DEVELOPMENT IN OCHROMONAS DANICA The Journal of cell biology, 1962
- THE EFFECT OF LIGHT INTENSITY AND SUCROSE FEEDING ON THE FINE STRUCTURE IN CHLOROPLASTS AND ON THE CHLOROPHYLL CONTENT OF ETIOLATED LEAVESThe Journal of cell biology, 1962
- Effect of light on growth of black valentine bean plastidsBiochimica et Biophysica Acta, 1961
- COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARISPlant Physiology, 1949