Determination of Pindolol in Plasma and Urine by High Pressure Liquid Chromatography

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Abstract
A high pressure liquid chromatography (HPLC) using an UV absorption detector was examined for the determination of pindolol in the plasma and urine. The pindolol extracted from biological fluid and the disopyramide used as an internal standard, were chromatographed as ion pairs with heptanesulfonic acid using a Nucleosil 7C18 reverse phase column. The coefficients of variation for the peak height ratio were 2.7% (50 ng/ml) to 4.3% (10 ng/ml) in the plasma and 2.3% (20 .mu.g/ml) to 3.8% (5 .mu.g/ml) in the urine. The detection limits of pindolol were .apprx. 2 ng/ml in the plasma and 0.5 .mu.g/ml in the urine. The plasma levels and the urinary excretion rates of pindolol after oral administration of 10 mg pindolol to a healthy male volunteer could be measured. This method is more sensitive and reproducible than the fluorometric method and may be used for a clinical pharmacokinetic study and a bioavailability study of pindolol.

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