Development of Anti-Interference Enzyme Layer for α -Amylase Measurements in Glucose Containing Samples
- 1 January 1983
- journal article
- research article
- Published by Taylor & Francis in Analytical Letters
- Vol. 16 (12) , 877-890
- https://doi.org/10.1080/00032718308065226
Abstract
A glucose oxidase-glucoamylase-bienzyme electrode has been developed and tested for determination of α-amylase activity. To eliminate interfering endogeneous glucose a glucose oxidase-catalase anti-interference layer was coupled with the bienzyme electrode. Linearity was obtained for the kinetic signal up to 1.0 I.U. α-amylase. Glucose was effectively eliminated up to 2 mM final concentration thus not influencing α-amylase determinations. A general concept for anti-interference enzyme layers is suggested.Keywords
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