Self Reactive Repertoire of Tight Skin Mouse: Immunochemical and Molecular Characterization of Anti-Topoisomerase I Autoantibodies
- 1 January 1991
- journal article
- research article
- Published by Taylor & Francis in Autoimmunity
- Vol. 9 (2) , 109-117
- https://doi.org/10.3109/08916939109006746
Abstract
Tight skin (TSK) mice develop cutaneous hyperplasia accompanied by histopathological alterations of skin and collagen metabolism similar to those described in human scleroderma. Diffuse scleroderma, the most severe form of progressive systemic sclerosis, is associated with the production of autoantibodies specific for Scleroderma 70 antigen (topoisomerase I). Our studies show that there is an increase in the level of serum anti-topoisomerase I (topo I) autoantibodies in aged TSK mice. The monoclonal antibodies isolated from TSK mice bind to epitopes which interact with autoantibodies from scleroderma patients. A significant number of TSK monoclonal anti-topo I antibodies and serum immunoglobulin (Ig) from aged TSK mice bear a cross reactive idiotype (Id) recognized by a syngeneic monoclonal anti-Id antibody obtained from a 2 month-old TSK mouse. Analysis of V gene usage by monoclonal anti-topo I antibodies showed that the majority of these antibodies are encoded by VH genes derived from VHJ558 family pairing with VK genes from various families in a stochastic manner.Keywords
This publication has 24 references indexed in Scilit:
- Genes regulating rheumatoid factorsSpringer Seminars in Immunopathology, 1988
- Specificities and V genes encoding monoclonal autoantibodies from viable motheaten mice.The Journal of Experimental Medicine, 1988
- Biased usage of certain Vk gene families by autoantibodies and their polymorphism in autoimmune miceMolecular Immunology, 1988
- Characterization of the antigen reactive with anti‐scl‐70 antibodies and its application in an enzyme‐linked immunosorbent assayArthritis & Rheumatism, 1988
- Autoantibodies of various specificities encoded by genes from the VH J558 family bind to foreign antigens and share idiotopes of antibodies specific for self and foreign antigens.The Journal of Experimental Medicine, 1987
- High Titers of Autoantibodies to Topoisomerase I (Scl-70) in Sera from Scleroderma PatientsScience, 1986
- Anti-immunoglobulin antibodies VI. Age-dependent isotype and auto anti-immunoglobulin variation during secondary immune response in 129 miceMechanisms of Ageing and Development, 1985
- Scl-86, a marker antigen for diffuse scleroderma.Journal of Clinical Investigation, 1985
- Idiotype-antiidiotype regulation. IV. Expression of common regulatory idiotopes on fructosan-binding and non-fructosan-binding monoclonal immunoglobulin.The Journal of Experimental Medicine, 1983
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979